The objective of this proposal aims at deciphering the mechanism of action of the recently discovered type IX secretion system (T9SS). The T9SS is responsible for Porphyromonas gingivalis pathogenesis through secretion of virulence factors, and for Flavobacterium johnsoniae motility through secretion of adhesins. Among the 18 identified proteins that are involved in T9SS function, the K-L-M-N proteins are supposed to form the core complex. We propose that the outer membrane ring-shaped K-N complex serves as an interaction platform to recruit the inner membrane L-M complex, and that the L-M complex is a novel rotary motor driven by proton motive force. In this project, we will solve the structures of the isolated soluble domains of L and N proteins, and of the L-M and K-L-M-N membrane complexes from the two bacterial models, by X-ray crystallography and cryo-EM. The function of the L-M complex, and notably its role as a rotary motor, will be addressed by in vivo approaches.
We are pleased to welcome Laura Schmitt, who joined the team as engineer to work with Adeline Goulet. Welcome Laura !
Vincent Delauzun’s thesis defence, Extracellular vesicles: an ideal tool for the study of membrane proteins, will be broadcast on the zoom platform.
ID de réunion : 942 7107 8490
Code secret : 1234
You want an appointment to talk about your project ? Go to the event’s website.
Feel free to read the article just here : https://fr.calameo.com/read/005154450e360a49aa8bd
We are pleased to welcome Marion and India, who joined the team as engineers to work on the Covid-19 project. Welcome girls !
You can visit the new website of our platform NabGen Technology to learn more about our activities.
La société des membres de la Légion d’Honneur de l’Orne soutient notre projet sur le COVID-19.
Un grand merci à eux !
If you want to learn more about Covid-19 and its genome, listen to Isabelle Imbert’s latest interview on the CNRS playlist #COVID19 La parole à la science (the word to science).
https://soundcloud.com/cnrs_officiel/genome-du-coronavirus?in=cnrs_officiel/sets/covid19-parole-a-la-science (French interview)
Our review entitled « Diversity of molecular mechanisms used by anti-CRISPR proteins: the tip of an iceberg?”is now online and can be accessed via the Biochemical Society Transactions website
You can listen to Isabelle Imbert’s new interview about the Covid-19 outbreak on the France Culture website (French interview).
You can listen (or listen again) Isabelle Imbert’s interview about the Cryo-EM structure of the Covid-19 spike protein (French interview).
You can go to read Isabelle’s latest interview in the “vrai ou fake” Franceinfo section : Is a bat bite in the laboratory the cause of the epidemic? (French interview)
Alain Roussel, Béatrice Amigues, Anaïs Gaubert and Lycia Die Morini were in CNRS Campus of Gif-sur-Yvette to take part in the 5th Frisbi User Meeting.
Rachel Morin-Pelchat, from the Sylvain Moineau lab at Université Laval (Québec, Canada), is visiting us for 3 months.
Our manuscript entitled “Versatile and robust genome editing with Streptococcus thermophilus CRISPR1-Cas9” is now online and can be accessed via the Genome Research website.
Our manuscript entitled “Allosteric inhibition of CRISPR-Cas9 by the anti-CRISPR protein AcrIIA6” is now online and can be accessed via the Molecular Cell website.